Analysis Note

ControlA549 cell lysate

Application

Anti-Annexin A2 is an antibody targeting the Annexin A2 protein, validated for use in WB & IHC.

Research CategoryApoptosis & Cancer

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Annexin A2 in human and rat colon tissues.

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Annexin A2 is known by several other names including: Annexin II, Calpactin I heavy chain, Chromobindin-8, Lipocortin II and Placental anticoagulant protein IV. Annexin A2 is a calcium-regulated membrane-binding protein that binds two calcium ions with high affinity. Annexin A2′s affinity for calcium is enhanced by anionic phospholipids. Annexin A2 has been shown to bind to human cytomegalvirus (HCMV) and may be involved in heat-stress response and apoptosis.

Immunogen

Epitope: Annexin 1 repeat domain

KLH-conjugated linear peptide corresponding to Annexin 1 repeat domain of human Annexin A2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Annexin A2 in 10 µg of A549 cell lysate.

Specificity

This antibody recognizes the Annexin 1 repeat domain of Annexin A2.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~36 kDa observed